Human airway epithelial hae cell line cufi 1 was derived from lung of a 14 year old patient with cystic fibrosis by dual retroviral infection with hpv 16e6 e7 lxsn pubmed. Add 6 0 to 8 0 ml of complete growth medium and aspirate cells by gently pipetting. A serum free method for culturing normal human bronchial epithelial cells at clonal density.
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