Lung Epithelial Cell Line A549

Human lung epithelial cells a549 which were bought from shanghai institutes for biological sciences.

Lung epithelial cell line a549.

Through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58 year old caucasian male. Ccl 185 a549 lung carcinoma homo sapiens 58 years caucasian the cells are positive for keratin by. Cross references nucleotide genbank. Human caucasian lung carcinoma cell line description derived from a 58 year old caucasian male.

Lung cancer and normal cell lines. In a study to. Cells from the human lung epithelial carcinoma cell line a549 atcc ccl 185 were maintained in dulbecco s eagle s medium supplemented with penicillin 0 6 μg ml 1 streptomycin 60 μg ml 1 glutamine 2 mm hepes buffer ph 7 4. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway.

A549 cells are hypotriploid alveolar basal epithelial cells. A549 a cell line model of nsclc used to evaluate the role of ccl22 and il 37 in epithelial mesenchymal transition 3 toxicology studies cellular toxicity of carbon based nanomaterials was tested in calu 1 and other lung cancer cell lines 4. Small cell lung cancer homo sapiens 59 years caucasian the cells do not express vasopressin oxytocin. Cancer research the generation of epithelial models of thedistal lung for studies into airway function and disease and virology.

This adenocarcinomic cell line is categorized as a non small cell lung carcinoma nsclc which tends to. 20 mm and 10 fetal calf serum at 37 c. X54079 human mrna for heat shock protein hsp27. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

The cells are used as models for the study of lung cancer and the development of drug therapies against it. A continuous tumor cell line from a human lung carcinoma with properties of type ii alveolar epithelial cells. The a549 cell line was established in 1972 by d j. Lieber m et al.

A549 cells are adenocarcinomic human alveolar basal epithelial cells and constitute a cell line that was first developed in 1972 by d. Cell culture and seeding of a549. A549 cell line origin. The a549 cell line was isolated in 1973 from a pulmonary adenocarcinoma.

A549 cells were cultured in kaighn s modification of ham s f 12 f 12k medium atcc nci h23 nci h1299 and hcc827 cells were cultured in rpmi 1640. The cell line of a549 was cultured in atcc formulated modified f 12 k medium containing 10 fetal bovine serum 100 u ml penicillin and 100 u ml streptomycin sulfate. The a549 cell line is a suitable host for many human.